Culture Age, Growth Medium, Ultrasound Amplitude, and Time of Exposure Influence the Kinetic Growth of Lactobacillus acidophilus

The growth pattern of probiotics can be modified by changing their nutritional factors and physiological stage. Meanwhile, high intensity ultrasound (HIUS) employed to increase probiotics’ biomass. one-factor-at-a-time (OFAT) approach was investigate the influence medium (MRS broth, whole milk, skim milk), culture age (1 day 7 days old) parameters (time amplitude) on kinetic L. acidophilus. oldest (7 days) had a greater lag phase time reach end sigmoidal curve (Tmax) (p < 0.05) as well lower rate (maximum potential μmax) compared youngest day). Regarding medium, milk presented greatest acidophilus counts 0.05). sonication times (60 90 s) change µmax Tmax. When 30% amplitude applied, μmax smaller Tmax were observed It concluded that age, Results from this study could used in design optimization processes improve probiotic at industrial scale.